Volume 7, Issue 3, May 2018, Page: 75-79
Expression and relationship of PPARγ and COX-2 in Peripheral Blood Mononuclear Cells of Multiple Organ Dysfunction Syndrome Patients
Xu Fei, Department of Emergency Medicine, 3201 Hospital of Xi'an Jiaotong University Health Science Center, Hanzhong, China
Qiao Wanhai, Department of Emergency Medicine, Second Affiliated Hospital of Xi'an Jiaotong University Health Science Center, Xi’an, China
Wang Liming, Department of Emergency Medicine, Second Affiliated Hospital of Xi'an Jiaotong University Health Science Center, Xi’an, China
Received: Jun. 5, 2018;       Accepted: Jul. 12, 2018;       Published: Aug. 8, 2018
DOI: 10.11648/j.cmr.20180703.14      View  359      Downloads  31
Abstract
To study the expression and relationship of cyclooxygenase-2(COX-2) and peroxisome proliferators activated receptor-γ (PPARγ) in peripheral blood monouclear cells (PBMCs) of multiple organ dysfunction syndrome patients. In the emergency department and intensive care unit (ICU) of our hospital, 60 MODS patients were divided into three groups with 20 cases in each group. They were MODS1 group (APACHE II score 0-10 points), MODS 2 groups (APACHE II score 11-20 points), MODS 3 groups (APACHE II score> 20 points) and control groups of 20 healthy subjects. Venous blood were sampled 1d, 3d, 6d after diagnosis of MODS, reverse transcriptase -polymerase chain reaction (RT-PCR) were used to detect the expression of PPARγ, COX-2 in PBMCs. The expression of PPARγ, COX-2 in the same period were compared between the MODS groups and the healthy control group. The study fond that the expression of COX-2 and PPARγ were low in vehicle control group. In MODS1 group the expression of PPARγ and COX-2 was higher than in vehicle control group; during the treatment period, PPARγ was significantly increased (P <0.05), but the expression of COX-2 was significantly lower (P <0.05). PPARγ expression in MODS 2 group and MODS 3group was lower than that in vehicle control group, and its expression was progressively decreased during the treatment (P <0.05);in contrast, Expression of COX-2 in MODS2 and MODS3 group was higher than that in vehicle control group, and its level was progressively increased during the treatment (P <0.05). The study revealed a significant negative correlation between COX-2 and PPARγ in MODS1 group, MODS2 group and MODS3 group. (MODS1 group r=-0.761, P<0.01; MODS2 group: r=-0.782, P<0.01; MODS3 group: r=-0.791, P<0.01). There was no significant correlation in vehicle control group (r = -0.185, P >0.05). Thus, PPARγ may protect the MODS patients by repressing the expression of cox-2 therefore it can be used as an important indicator and potential target to determine the condition and treatment of MODS.
Keywords
Multiple Organ Dysfunction Syndrome (MODS), Peroxisome Proliferator-Activated (PPARγ), Receptors-γ, Cyclooxygenase-2 (COX-2)
To cite this article
Xu Fei, Qiao Wanhai, Wang Liming, Expression and relationship of PPARγ and COX-2 in Peripheral Blood Mononuclear Cells of Multiple Organ Dysfunction Syndrome Patients, Clinical Medicine Research. Vol. 7, No. 3, 2018, pp. 75-79. doi: 10.11648/j.cmr.20180703.14
Copyright
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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